The role of PLS3 in osteogenic differentiation and osteocyte transcriptome signature

Plastin-3 (PLS3) encodes T-plastin, an actin-bundling protein mediating the formation of actin filaments by which numerous cellular processes are regulated. Loss-of-function genetic defects in PLS3 are reported to cause X-linked osteoporosis and childhood-onset fractures. However, the molecular etiology of PLS3 remains elusive.The Pls3 knockdown murine MLO-Y4 cell line was employed to provide insights in global gene expression. Our findings in different bone cell types indicate that the mechanism of PLS3-related pathology extends beyond actin-bundling proteins implicating broader pathways of bone metabolism.