RNA-seq of mouse tissue samples from animal model : Effects of GSK3 on gene expression

In the present study, RNA-seq technique were performed To explore biological significance of GSK3 in the neuro-cutaneous signaling network of chronic itch.Five repetitive mRNAs were deeply sequenced using MGISEQ-2000 to generate an mRNA profiles. We found that when using GSK3's inhibitor SB415286 AD mouse model or non GSK3's inhibitor SB415286 AD mouse model for high-throughput sequencing, using GSK3's inhibitor SB415286 ACD mouse model or non GSK3's inhibitor SB415286 ACD mouse model for high-throughput sequencing，SB415286 affected the expression of a variety of itching factors and skin inflammatory factors related to the skin pathology of human chronic dermatitis. These changes may indicate that GSK3 is closely related to inflammation and pruritus. This finding might help us to better understand the immune-neuro-modulatory mechanism in itch circuits. Mouse ear tissue were from SB415286-injected AD model mice (0.7mg/kg/mice iintraperitoneal injection) or vehicle control，mouse ear tissue were from SB415286-injected ACD model mice (2mg/kg/mice iintraperitoneal injection) or vehicle control，The gene expression of these cells were investigated utilizing MGISEQ-2000.