Whole-genome transcriptomic analysis of Notch1-expressing cells in mouse intestinal tumours

To define and compare the genome-wide transcriptional signatures of Notch1+ cells in intestinal tumors and in normal ISCs we performed Affymetrix analyses of these two populations. Three replicates from a pool of 5-7 mice (tumors) and 2 mice (normal ISCs) were used for the analysis. GFPpos and GFPneg tumor and normal ISCs were sorted from the same replicate. A total of 12 samples were analyzed (6 per condition).