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Figure A. Purity and identification of Botulinum toxin A preparation.

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Figshare2015-12-03 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Botulinum_Neurotoxin_Type_A_Induces_TLR2_Mediated_Inflammatory_Responses_in_Macrophages_/1372085
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Endotoxin free-BoNT/A have been purified by Superdex 200 (A) and affinity chromatography (B) as described in materials and methods. Superdex 200 FPLC preparation showed BoNT/A heavy chain (Hc), light chain (Lc), NTNH, and haemagglutinins on SDS-PAGE analysis. Affinity chromatography showed only Hc and Lc chains with no other contaminants. Hc (C) and Lc (D) were identified by peptide mass fingerprint analysis. (PDF)
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