Figure A. Purity and identification of Botulinum toxin A preparation.
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Endotoxin free-BoNT/A have been purified by Superdex 200 (A) and affinity chromatography (B) as described in materials and methods. Superdex 200 FPLC preparation showed BoNT/A heavy chain (Hc), light chain (Lc), NTNH, and haemagglutinins on SDS-PAGE analysis. Affinity chromatography showed only Hc and Lc chains with no other contaminants. Hc (C) and Lc (D) were identified by peptide mass fingerprint analysis. (PDF)
创建时间:
2015-12-03



