Absolute quantification of budding yeast proteome with PCS method

Absolute quantification of proteome is one of the most important tasks in proteomic research. The aim in this analysis is providing proof-of-principle of our originally developed strategy for absolute quantification of multiple proteins present at various concentrations in a cell, using stable isotope-labeled peptide concatenated standard (PCS). This study also provides insights into structures of regulation of metabolic pathway and into biological mechanisms for regulation of expressed protein abundance.