High throughput phenotypic screen and transcriptional analysis identify new compounds, targets and pathways for macrophage reprogramming II. High throughput phenotypic screen and transcriptional analysis identify new compounds, targets and pathways for macrophage reprogramming II

Purpose: To understand compound effects on human macrophage repolarization in the transcriptional level Overall design: Fresh isolated human blood monocytes were cultured with 50ng/mL M-CSF for 7 days to generate M0 status macrohages. Macrophages were differentiated into M1 macrophages (M1 Mac) by 50ng/mL IFNg plus 50ng/mL TNFa or M2 macrophages (M2 Mac) by 10ng/mL IL4 plus 10ng/mL IL13. M2 Mac were treated with different M1-type compounds or IFNg or DMSO for 24 hrs. M1 Mac were treated with different M2-type compounds or IL4 or DMSO for 24 hrs. RNA was isolated and purified by Qiagen Rneasy micro kit and applied to RNAseq.