The effect of OsWOX4 knockdown on the expression profile in rice the leaf primordia and the SAM

We used an inducible knockdown system, in which the OsWOX4 expression is silenced by the application of dexamethasone (DEX) using a pACT1-GVG>OsWOX4:RNAi construct. To compare gene expression profiles between mock and DEX treated plants, we performed microarray analysis, using Rice (US) gene 1.0 ST array. After 3 or 12 h of DEX treatment of 5-day-old plants, the shoot apices including the SAM and leaf primordia, which contained the whole part of P1 and P2, most part of P3, and the basal parts of P4 and P5, were harvested.