Whole-exome sequencing analysis of primary tumors and metastases from the NPK (Nkx3.1CreERT2/+; Ptenflox/flox; KrasLSL-G12D/+; R26R-CAG-LSL-EYFP/+) prostate cancer mouse model

Whole-exome sequencing analysis of lineage-marked prostate primary tumors and metastases from the NPK mouse model (Nkx3.1CreERT2/+; Ptenflox/flox; KrasLSL-G12D/+; R26R-CAG-LSL-EYFP/+) Male mice of a a predominantly C57BL/6 background were induced to form tumors at 2-3 months of age by administration of tamoxifen using 100 mg/kg (in corn oil) once daily for 4 consecutive days. Following tamoxifen-induction, mice were monitored daily and euthanized when their body condition score was <1.5, or when they experienced body weight loss >20% or signs of distress, such as difficulty breathing or bladder obstruction. Whole exome sequencing was done on matched trios of primary tumors, lung metastases, and bone metastases, as well as tails (as a control) from five independent mice.