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Comparative Chloroplast Genome Analyses Among Three Sympatric Species of Atraphaxis

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DataCite Commons2025-04-27 更新2025-05-18 收录
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DNA was extracted using an improved CTAB method and stored in a refrigerator at -20 ° C. After the DNA samples pass the test, the Covaris ultrasonic crusher is used to randomly break the qualified DNA samples; The preparation of the library was completed through multiple steps such as end repair, addition of A-tail, addition of sequencing connectors, purification, and PCR amplification; After quality inspection, the Illumina Hiseq 2000 platform of Beijing, China was used to sequence the entire genomes of three species, namely Polygonum spinosum, Polygonum frontalis, and Polygonum slender.
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创建时间:
2023-06-30
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