Preference responses of sexually mature female sea lamprey to mixtures of DKPES:3 KPZS administered to stream nests.

Treatments include: Control (synthesized 3 KPZS 5×10−13 M applied to both nests), and three separate ratios (v:v) of 3,12-diketo-4,6-petromyzonene-24-sulfate (DKPES) and 3-keto petromyzonol sulfate (3 KPZS) applied to one nest (Treatment nest), while an equal concentration of 3 KPZS alone (5×10−13 M) was applied to the adjacent nest. Treatment nests and control nests were alternated per trial. N refers to the total number released for each treatment. Upstream refers to the percentage of released animals that moved upstream and entered a nest. Treatment nest refers to the percentage of animals that moved upstream and then entered the nest activated with each treatment. Distribution data were evaluated with logistic regression. Treatments that share a letter are not significantly different (two-tailed t test; α = 0.05).