Next Generation Sequencing of miRNome of G-CSF treated huCD34+ HSPCs

MicroRNAs (miRNAs) are a class of small non coding RNAs (ncRNAs) with 19-25 nucleotides in length, which can inhibit the translation or induce the destabilization and/or degradation of their mRNA targets, usually by binding in an incomplete manner to the 3`untranslated region (3`UTR) of their respective targets. We were interested in miRNAs that are regulated during G-CSF induced myeloid differentiation. Overall design: Examination of miRNA expression patterns upon G-CSF administration on huCD34+ HSPCs. NGS was performed out of 3 independent experiments.