Table 2 in Identification of a UDP-Glucosyltransferase favouring substrate- and regiospecific biosynthesis of flavonoid glucosides in Cyclocarya paliurus

Table 2 Effects of various ions on the enzymatic activity of recombinant C. paliurus GT 1.
ReagentConcentrationRelative activityIC50 (mM)(mM)(%)Control–100–NaCl50099.35 ± 2.47NDNa2SO450096.77 ± 3.83NDKCl598.12 ± 4.11NDMgCl25135.36 ± 2.98–CaCl2562.68 ± 2.556.93 ± 0.31CuSO459.13 ± 1.040.94 ± 0.04FeSO45121.15 ± 4.65–FeCl3550.14 ± 2.425.50 ± 0.83ZnCl2572.33 ± 2.908.00 ± 0.50NiCl2597.98 ± 4.52NDDimethyl sulphoxide( v/2098.98 ± 3.67NDv, %)Methanol ( v/v, %)2096.22 ± 2.47NDβ- mercaptoethanol (β-598.41 ± 3.30NDME)Dithiothreitol (DTT)593.75 ± 4.13ND
ND: no effects detected at the selected concentrations.