The start (ATG) and stop (CTA) codons are indicated in bold italics. The coding sequences of the cDNAs are indicated in italics. The sequence coding for the Strep-tag II is underlined. The restriction

NIAID Data Ecosystem20

1.1_Data for pET28b(+)-SUMO_p53_allconst_raw.

重组蛋白表达构建

大肠杆菌转化实验

Raw data for M1.1_ Gene design and E. coli transformation.

Zenodo2025-01-06 更新10

Restriction Endonucleases for the Construction of pcDNA3.1 B, pEGFP-N1, pBiFC-VC155 and pBiFC-VC173 Vectors.

基因克隆

蛋白质表达载体构建

+: restriction endonucleases used to cleave the PCR products.

NIAID Data Ecosystem20

Primer sequences used for amplification.

分子克隆引物设计

蛋白质表达载体构建

Underlined restriction enzyme sites - CATATG: NdeI, AAGCTT: HindIII, GGATCC: BamHI, GAATTC: EcoRI, ACGCGT: MluI, ACTAGT: SpeI, TTAATTAA: PacI, CTCGAG: XhoI. C1 and C2 are alternate primers for cloning

Figshare2015-12-02 更新20

1.1_Data for pET28b(+)-SUMO_p53_allconst_raw.

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相关数据集