Methylome analysis of a isogenic line of Atlantic Salmon
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https://www.ncbi.nlm.nih.gov/sra/SRP281943
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资源简介：
WGBS on gonads from isogenic and normal diploid female salmon.In October 2017 gonads from 4 female diploid and 4 female isogenic fish from fish line L3 (average whole fish weight 35g) were sampled. Genomic DNA was extracted using Qiagen Blood and Tissue kit. Whole-genome bisulfite sequencing (WGBS) libraries were prepared from 500ng of genomic DNA using the method SPlinted Ligation Adapter Tagging (SPLAT) method (https://pubmed.ncbi.nlm.nih.gov/27899585/). The resulting dual-indexed SPLAT libraries were sequenced together on a lane of NovaSeq S4 as 2x150 bp reads. Sequencing was done together with a bacteriophage PhiX DNA spike-in.
创建时间：
2020-09-10
