Survival of different <i>Y. pseudotuberculosis</i> strains inside macrophages, in the presence or absence of Rac inhibitor NSC23766.

BMDMs were infected with the indicated strains at an MOI of 10 as described in Figure 1. When indicated, 100 µM NSC23766 was present throughout the experiment. (A–B) Intracellular bacterial survival was determined by CFU assay, as described in Figure 1. Results shown are the means from three independent experiments with duplicate infection wells. Error bars show standard deviations. ***, P<0.001, comparing each strain with to without Rac inhibitor treatment individually, as determined by one-way ANOVA. (C) Intracellular survival of mCherry-encoding IP6 was determined by fluorescence microscopy, as described in Figure 7C. The left panel shows mCherry or an overlay of mCherry and phase contrast signal at 24 h post infection from representative images. The right panel shows percentage of mCherry positive macrophages quantified from three independent experiments. Error bars show standard deviations. ***, P<0.001 determined by t-test.