Plant parasitic nematodes single embryo RNA sequencing

In order to study the similarities and differences in embryonic development between plant-parasitic nematodes and free-living nematodes, we performed RNA-seq on embryos of three plant-parasitic nematodes at a total of 11 stages from the single-cell stage to the J1 stage We recorded the embryonic development process of Meloidogyne incognita, Heterodera glycines, and Ditylenchus destructor species, and constructed transcriptome datasets of single embryos of these three species utilized low-input smart-seq2 RNA-seq technology. The datasets encompassed 11 complete embryonic development stages, which are Zygote, 2-cell, 4-cell, 8-cell, 24-44 cell, 64-78 cell, Comma, 1.5-fold, 2-fold, Moving, L1, which each stage generated four to five replicates. a total of 162 high-resolution transcriptome libraries were obtained.