Generation of intestinal progenitor cells from mouse fibroblasts. Mus musculus

We show direct conversion of mouse fibroblasts to cells that closely resemble intestinal stem cells (ISCs), through the state of fetal-type progenitor cells, called FIPCs. The induced ISCs (iISCs) exhibit self-renewal capacity and intestinal multi-lineage differentiation potential. Upon transplantation, iFIPCs and iISCs reconstitute colonic and intestinal epithelia, respectively. Overall design: We conducted comparative global gene expression analyses among MEFs, iHep cells, iISC-derived budding organoids, Crypt-derived budding organoids, iFIPC-derived spherical organoids and budding organoids, and FI-derived spherical organoids and budding organoids.