SWAMP eDNA Metabarcoding Monitoring and Analysis Project (SeMMAP) Data Resource Hub

The SWAMP environmental DNA Metabarcoding Monitoring and Analysis Project (SeMMAP) was created to explore the use of eDNA metabarcoding for surface water quality monitoring and how this method may achieve the project’s goals of monitoring more with less resources and integrating SWAMP programs through a single data source. We are creating a data management plan and exploring the benefits of collaborating with volunteer and Tribal partners to collect the same quality data as our regional and internal SWAMP programs. eDNA metabarcoding is extremely cost effective compared to traditional methods and the simple collection process allows almost anyone to collect robust scientific data. The project's aim is to bring inclusion to the Water Boards by putting monitoring into the hands of those who have been marginalized by the Boards and other government agencies, while simultaneously outsourcing some of the more costly aspects of sampling in order to save taxpayer money and deliver high quality data. In short, "Science for the Stewards!" We operate on the principles of Equity, Accountability and Transparency. To this effort, we have created the SeMMAP Data Resource Hub. The SeMMAP Hub will serve as a site for external partners, regional partners, SWAMP program personnel, decision makers and the public to view the pace and direction of the project. It also provides access to the raw data collected as well as data visualization tools to help users analyze and interpret the DNA sequencing results. The SeMMAP Hub contains data dashboards, maps, and a partner project gallery for participants to share and view how they’re using their eDNA data to answer scientific questions and monitor water quality. The Hub also includes illustrated instructions for sample collection, guides on best practices, literature to help with study design, as well as the raw data and metadata standards. The maps within display the 2020 and 2021 results of SeMMAP sampling efforts using Jonah Ventures aquatic eDNA kits. Included are the locations, field measures and MiFish and 23S sequencing results of eDNA sampling for fish and phytoplankton communities respectively. 45 samples were taken between June 2020 and October of 2021, at 30 unique sites. This content was created by Peter Houpt from the State Water Resources Control Boards, SWAMP eDNA Metabarcoding Monitoring and Analysis Project. [e-mail], [e-mail]