Angptl-7 knockdown RNA sequencing of 4T1 orthotopic transplants into rat mammary fat pads

These samples are derived from tissues obtained from 4T1 spheroids transplanted orthotopically into the mammary fat pads of SRG OncoRats. 4T1 cell lines were established by viral transduction that stably express either a non-targeting control shRNA (NT4) or an shRNA targeting Angptl-7. 4T1 cells were cultured as spheroids in suspension for 24-hours prior to transplantation in 2% matrigel. 600,000 cells in a 1:1 mixture of matrigel and DMEM/F12 were transplanted orthotopically into the right T4 fat pads of SRG rats. At day 27 post transplanation, primary tumors. The primary tumors were cut in half to expose the cross-section, and the tumors were dissected to isolate the necrotic core region and the non-necrotic rim region. Samples were snap frozen and sent to Genewiz-Azenta for RNA extraction, library prep, and next-gen sequencing. 4T1 cells transduced with either non-targeting control shRNA (n=4 animals) or Angptl7 knockdown shRNA (n=3 animals) were transplanted into the mammary fat pads of SRG rats. Tumors were dissected into necrotic core and non-necrotic rim regions, and regions were separately submitted for RNA-sequencing.