Raw diffraction images of YidC (phasing data by Hg-MAD)
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Diffraction images of mercury-bound <em>Bacillus halodurans</em> YidC mutants for Hg-MAD phasing. This phase information was used for PDB entries 3WO6 and 3WO7 (Kumazaki et al. 2014). All data were collected from loop-harvested crystals on BL32XU, SPring-8 at wavelengths of 1.00000 Å (peak) or 1.00945 Å (edge) using the MX225HE CCD detector. From the M146C mutant, helical (360 or 720°/crystal, 2°/frame) datasets were collected using 10×1 μm<sup>2</sup> beam. From a Y150C mutant, a helical (360°/crystal, 2.5°/frame) dataset was collected using 15×1 μm<sup>2</sup> beam, which was used for phase determination. The crystals belonged to space group <em>P</em>2<sub>1</sub> with unit cell parameter a~44, b~60, c~60 Å, β~100°.
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Zenodo创建时间：
2019-03-29
