Verification of assay on amplified libraries with shotgun sequencing data.

1As discussed in the methods section, the assay percentages are meant as a guide to compare samples and are not expected to match the absolute values yielded via shotgun sequencing. 2The scaled shotgun sequencing results do not include reads without BLAST matches or reads which matched higher taxonomic levels (e.g., eukaryotes or metazoa). 3N/A indicates the primer set did not fluoresce before the negative control for the sample.