scRNAseq of immune cells from young and aged mouse bladders

Aging has multifaceted effects on the immune system, but how aging affects tissue-specific immunity is not well-defined. Bladder diseases characterized by chronic inflammation are highly prevalent in older women, but mechanisms by which aging promotes these pathologies remain unknown. Tissue transcriptomics of unperturbed, young, and aged bladders identified a highly altered immune landscape as a fundamental feature of the aging female bladder. Detailed mapping of immune cells using single cell RNA- sequencing revealed novel subsets of macrophages and dendritic cells and unique changes to the immune repertoire in the aged bladder. B and T cells are highly enriched in aged bladders and spontaneously form organized bladder tertiary lymphoid tissues (bTLTs). 3 young (3 mo) and 1 aged (18 mo) C57BL6J mouse bladders衰老对免疫系统具有多方面的影响，但衰老如何调控组织特异性免疫的机制尚未明确。以慢性炎症为特征的膀胱疾病在老年女性中患病率极高，但衰老促进此类病理发生的具体机制仍未阐明。对未受干预、年轻及衰老小鼠膀胱开展的组织转录组学分析显示，免疫景观发生显著改变是衰老雌性膀胱的核心特征。通过单细胞RNA测序（single cell RNA sequencing）对免疫细胞进行精细分型绘制，揭示了衰老膀胱中巨噬细胞与树突状细胞的新型亚群，以及免疫组库的独特变化。B细胞与T细胞在衰老膀胱中显著富集，并可自发形成结构完整的膀胱三级淋巴组织（bladder tertiary lymphoid tissues，bTLTs）。本研究涉及的实验样本包括3只年轻（3月龄）及1只衰老（18月龄）C57BL/6J小鼠的膀胱组织。