Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Imaging modality / instrument: <em>Brightfield</em> + <em>Slimfield</em> Image format:<em> OME TIFF (16 bit) + MicroManager metadata files</em> Microscope settings: <em>488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS</em> Samples and acquisitions: Fluorescent fusions in live E.coli cells. MMC = mitomycin C No. fields of view MMC- MMC+ (0.5 ug/ml 3h) RecA-mGFP 7 15 RecB-sfGFP 17 21 MG1655 control 10 10 Approx. size before/after compression: 21 GB / 7 GB