FRET_measurement

Measurement of FRET ratio from MDA-MB-231 cells stably transfected with either glucose (FLII12Pglu-700uDelta6) or lactate (Laconic) reporter.<br>fretx_0.csv: confocal microscope image with no glucose; before addition of glucose to x mM (x = 0.5, 1, 2, 5, 8, 10, 15)fretx_x.csv: confocal microscope image after addition of glucose to x mM (x = 0.5, 1, 2, 5, 8, 10, 15)<br><br>calibration_xxx.csv: normalized FERT ratio of glucose/lactate in protein extraction(vitro)/intact cell(vivo) in response to glucose/lactate concentration; first column: concentration<br>longitudinal_xxx.csv: longitudinal measurement of normalized FRET ratio with different initial glucose/lactate concentrations;first column: concentration; first row: time<br>lac_colc2.csv: FRET response of lactate reporter to therapy with CoCl2xxx_CytochalasinB: FRET response of glucose/lactate reporter to therapy with Cytochalasin Bxxx_Phloretin: FRET response of glucose/lactate reporter to therapy with Phloretin<br>