Microarray analysis of Replicative senescence and TNF-α induced senescence in Human umbilical vein endothelial cells (HUVECs)

Cellular senescence is a dynamic tumor suppression mechanism that limits the proliferation of impaired cells, by executing a stable cell cycle arrest. Understanding the molecular pathways and regulatory circuits that are involved in the process of senescence is presently incomplete. In this study, we determined the changes in gene expression during the establishment of replicative senescence, by comparing the expression profiles of young and senescent human umbilical vein endothelial cells (HUVECs). Exploration of array data using ingenuity pathway analysis showed that genes involved in cell cycle regulation, cellular assembly and organization, DNA replication, recombination and repair were significantly down regulated during senescence. Human umbilical vein endothelial cells (HUVECs) were cultured continously until cells exhibit senescent morphology or reached proliferative arrest (Replicative senescence) or cells were untreated or treated TNFα (5ng/ml) continously for 26 days or (Passage 2 -Passage 8/ Passage 3- Passage9)//until cells exhibit senescent morphology. Gene expression of young cells were compared to corresponding old passage or replicative senescence cell or with cells underwent senescence with inflammatory cytokine TNF-α Some samples are missing CEL files due to storage failure.