Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

Uracil in DNA can be generated as a result of cytosine deamination or dUMP misincorporation. However, its distribution in the human genome is poorly understood, due to the lack of a sensitive detection method. Here we present “dU-seq”, a selective labeling and pull-down technology, to profile uracil in the human genome. dU-seq specifically labels uracil-containing DNA via an in vitro base excision repair reaction and identifies thousands of uracil peaks in the human genome. Overall design: dU-seq performed on three wild-type cell lines, one KO cell line and two 5-fluorodeoxyuridine treated cells; sample Titles with "biotin-dCTP" means in the dU-seq library preparation, the DNA labeling step utilized biotin-dCTP while other samples utilized biotin-dUTP; sample Titles with "5F" means 5-fluorodeoxyuridine treated cells.