Effect of SULF2 knockdown on Wnt/ß-catenin signaling in THCE cells.

THCE cells were transduced with either control shRNA or SULF2 shRNA1. TOP/FOP was determined for each cell type in 4 independent experiments as described in Materials and Methods. Mean values ±SEMs based on 3 replicate determinations are shown. The TOP/FOP value for control shRNA cells was normalized to 100% within each experiment, and the TOP/FOP signal for the SULF2 shRNA cells was computed relative to this normalized value (% Control). The mean ± SEM is shown (at the bottom right) for the 4 experiments. The normalized values differed between the control shRNA and SULF2 shRNA transduced cells with p = 0.007 by a two-tailed, paired Student t-test.