Rescue efficiency of PB2, PB1, PA reassortants between Bat09:mH1mN1 and PR8.
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*Rescue efficiency definition.Very easy (++++): P0 viral titer 106–108 TCID50/ml, or severe CPE observed in P1 within 1 dpi;Moderate (+++): P0 titer 104–106 TCID50/ml, or obvious CPE observed in P1 within 2 dpi;Difficult (++): P0 titer 102–104 TCID50/ml, or weak CPE observed in P1 within 4 dpi;Very difficult (+): P0 titer lower than 102 TCID50/ml, or CPE not observed until P2/P3;Negative (Neg): rescue failed, no CPE observed through passage 3.For each combination, the rescue was repeated at least 3 times.Rescue efficiency of PB2, PB1, PA reassortants between Bat09:mH1mN1 and PR8.
创建时间:
2015-12-02



