Gene expression analysis from placenta of Cornelia de Lange Syndrome (CdLS) mouse models

CdLS is caused by mutaion in cohesin and associated proteins, such as Nipbl and Hdac8. We performed RNA seq analysis from Nipbl+/- and Hdac8-/0 placenta with their respective wild type control at 14.5 dpc, to identify molecular pathways affected. To understand the effect of protein kinase R (PKR) on gene expression we also performed RNA seq analysis from Nipbl+/- and Hdac8-/0 placenta lacking PKR. Overall design: Total RNA was isolated using Trizol method and cDNA was prepatred using TruSeq Stranded Total RNA LT kit (RS-122-2302) for Illumina sequencing.