Droplet-based single-cell RNA-sequencing of mouse and naked mole-rat spleen and circulating immune cells, in natural conditions, following lipopolysaccharide (LPS) challanege, and saline control

Single-cell RNA-sequencing was performed in the mouse and naked mole-rat immune systems in order to map and compare their immune cell repertoirs and their cell-specific gene-expression responses to LPS challenge Overall design: Single-cell RNA-sequencing was obtained from: 1. 4 adult mouse and 4 adult naked mole-rat spleens in duplicate; 2. 4 adult mouse and 3 adult naked mole-rat circulating immune cells; 3. 2 adult mouse and 2 adult naked mole-rat spleens following LPS challenege in duplicate; 4. 2 adult mouse and 2 adult naked mole-rat spleens following saline administration in duplicate; 5. 2 adult mouse and 2 adult naked mole-rat circulating immune cells following LPS challenege in duplicate; 4. 2 adult mouse and 2 adult naked mole-rat circulating immune cells following saline administration in duplicate;