Sequence variations detected by pyrosequencing and Sanger sequencing- based genotypic DR test.

a: The categorization of frequencies based on the frequencies of variations identified by pyrosequencing;
bNumber of nucleotide and amino acid variation listed under Sanger sequencing is the sum of all variants identified in the Sanger sequences that were detected by pyrosequencing at the given frequency;
c: Absolute value of the difference in variation frequency of nucleotide (NT) or amino acid (AA) frequency between the two approaches for concordantly detected mutations (Mean± standard deviation).