Template modeling scores [TM] and root mean square deviation scores (RMSD) for the comparison of structural similarities between scFvs.

The higher the template modeling score [TM] and the lower the root mean square deviation score (RMSD) the more closely two structures are related. Comparison of two identical structures (D9 and S9/P2 each with itself) yields 1 for the TM score and 0 for the RMSD score. The TM score weighting algorithm is less sensitive to distant than to close matches and is therefore a more sensitive measure for structural similarity than the RMSD score [46].