The gene expression profiles of human bone marrow stromal cells derived from multiple myeloma patients

Bone marrow stromal cells (BMSCs) and their exosomes are a promising area of cancer therapy. Multiple myeloma (MM) is refractory hematologic malignancy. Bone marrow stromal cells (BMSCs) interact with MM cells in the bone marrow (BM), and also create a permissive microenvironment for MM cell growth and survival. Recent evidence indicated that exosome-mediated MM cell-BMSC communication plays an important role in the MM microenvironment. In this study, we investigated the biological property of the exosomes and exosomal miRNAs derived from BMSCs, aiming to establish the emerging strategies to target MM microenvironment to prevent tumor growth and spread. Human BMSCs of healthy donors were purchased from Lonza (Normal-BMSCs). BM samples were obtained from multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) patients in accordance with the Declaration of Helsinki and using protocols approved by the research Ethics Committee of Tokyo Medical University (IRB No. 2648), and BMSCs derived from MM patients (MM-BMSCs) and MGUS patients (MGUS-BMSCs) were isolated using the classical plastic adhesion method. BMSCs (2 × 10^4 cells) were transfected with -miR-10a mimic (Ambion), or scrambled control (Negative Control 1; Ambion) by using HiPerFect (Qiagen).Total RNA from each sample (Normal-BMSCs, MM-BMSCs and MGUS-BMSCs) was processed for hybridization onto GeneChip® human Gene 2.0 ST Array (Affymetrix) according to the manufacturer's protocol and scanned.