Gene expression analysis of yeast lacking Set4 under normal and stress conditions

Genome-wide gene expression data were generated for wildtype and set4 mutant yeast under aerobic and hypoxic conditions to determine the contribution of Set4 to gene expression. Illumina-based sequencing was performed on strand-specific libraries prepared from mRNA purified from yeast grown in rich medium aerobically or under hypoxia. Reads were processed for qualiy control, mapped to the genome, and differential gene expression was determined based on log fold-change differences between set4 mutants and wildtype. Three replicates each of wildtype and set4 mutant yeast grown in YPD medium, 30 degrees C either under aerobic or hypxic conditions are reported