Type 2 innate-like pathogenic function of PD-1high CD4 T cells aggravate pulmonary fibrosis

Single-cell RNA sequencing(scRNA-seq) was performed to investigate the differentiation process of steady-state CD4 T cells following treatmernt with IL-25 and IL-33, after initial isolation via FACS. This analysis revealed that steady-state PD-1high CD4 T cell differentiate into patogenic Th2 cells, characterized by the expression of IL-13, IL-5 and amphireguling. These findings suggest that steady-state PD-1high CD4 T cells may contribute to pulmonary fibrosis through its differntiation into pathogenic Th2 cells. This dataset includes previously published scRNA-seq data from [Min-Ji Cho et al., 2023]. CD44 high CD4 T cells from the spleen of C57BL/6J mice were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNA-seq.