Quantification of the phase shifts in <i>opn4xa</i>+/+ versus <i>opn4xa</i>-/- larvae kept in DD or submitted to a 2 hours pulse of white light at CT16.

As for Table 1, the Δphase is the difference between the phase of the two last cycles and the phase of the two first cycles. A Phase shift was observed in DD owing to the period that is close to 25 hours which generates a ~1 hour-shift every cycle. Phases were calculated with the FFT-NLLS method. Upon a pulse of light at CT16 or CT21 a statistical difference was observed between DD and pulsed ctrl larvae (****, p<0.0001 using a Mann-Whitney two-tailed test). In both phase delays (PD, pulse of light at CT16) and phase advance paradigms (PA, pulse of light at CT21), no statistical difference between opn4xa+/+ and opn4xa-/- was observed using a Mann-Whitney two-tailed test. For each type of paradigm (DD, PD and PA) three independent experiments were pooled.