AFF4 promotes tumor progression by modulating PTEN/PI3K/AKT/mTOR axis to accelerate glycolysis in lung adenocarcinoma

Bioinformatics analysis and western blotting were used to examine AFF4 expression in lung adenocarcinoma cells and tissues. The changes in cell proliferation, migration, invasion and apoptosis were determined by loss- and gain-of-function assays. Metabolite determination assays of glucose and lactate were used to evaluate the glycolysis level. Transcriptome sequencing, cleavage under targets (CUT)&Tag, dual-luciferase report assay and a series of rescue experiments were used to explore the underlying mechanisms. AFF4 Cleavage under targets and tagmentation sequencing (CUT&Tag-seq) were performed in A549 and PC9 cells