The Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma Revealed by Single-Cell Transcriptomics

Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with a high possibility of metastasis. The tumor microenvironment (TME) is well known to play an important role in LSCC metastasis. To gain insights into the TME of LSCC, we performed single-cell RNA-seq (scRNA-seq) using LSCC with lymph node metastases. We collected 10 fresh tissues immediately after surgery, including carcinoma in situ (T), normal laryngeal mucosal epithelia (N), margins of cancer (R), and lymph nodes (L). Single-cell suspension was prepared immediately and then subjected to 3’ droplet- based single-cell sequencing (10×Genomics)