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Results transcriptomes sequencing dataset for human melanoma and melanocytes cell cultures

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DataCite Commons2023-08-16 更新2024-07-13 收录
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https://uj.rodbuk.pl/citation?persistentId=doi:10.26106/y8e9-7603
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资源简介:
Results of totalRNA transcriptome sequencing for human melanoma: WM115, WM266-4, and melanocytes HEMa-LP cell lines in (*.xlsx) files format. The raw readings were mapped to the human reference genome GRCh38 from the Ensembl database (v. 96) using the Hisat2 v. 2.1.0 program (2). Cuffquant and Cuffmerge version 2.2.1 (3) and the GTF Homo_sapiens.GRCh38.96.gtf file from the Ensembl database were used to calculate the expression level of the transcripts. In the presented analysis, 131 621 transcripts were measured. A one-way ANOVA analysis was performed aimed at identifying transcripts with significantly different expression levels in at least one group experimental. The obtained p-values were corrected for multiple testing using the FDR algorithm For transcripts with significantly different expression levels in the studied groups (FDR from test ANOVA <0.1) post hoc analysis was performed with Student's t-test. Each group was compared with each, and the obtained p-values were corrected by the Bonferroni correction.
提供机构:
Jagiellonian University in Kraków
创建时间:
2021-04-21
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