Clariom D expression in breast cancer samples

We analysed expression using Clariom D array in 42 breast cancer FFPE samples The RNA for the Clariom™ D study was extracted from tissue fixed in formaldehyde and embedded in paraffin, preserved at room temperature. Four sections of 20 μm from breast cancer tumour paraffin embedded were used. The extraction of the RNA material was carried out using the commercial kit RecoverAll ™ Total Nucleic Acid Isolation Kit from ambion® (Applied Biosystems ™ by Life Technologies ™, Carlsbad, California, USA). RNA was quantified by Qubit® 3.0 (InvitrogenTM by Termo Fisher Scientific, Waltham, MA, USA) y Qubit® RNA HS Assay Kit (Molecular Probes® by Life Technologies Carlsbad, California, USA). Before hybridization RNA quality was assessed using Real Time PCR (qRT-PCR).