Bulk RNA sequencing of acetylcholine-synthesizing macrophages from subcutaneous adipose tissue

We aimed to study the transcriptomic profile of acetylcholine-synthesizing adipose macrophages. Using a ChAT-eGFP reporter mouse (ChAT, or choline acetyltransferase, is the main enzyme for acetylcholine biosynthesis) acutely exposed to cold temperature, we used FACS to isolate ChAT-eGFP+ and ChAT-eGFP- macrophages from inguinal subcutaneous white adipose tissue (IWAT). We subjected these cells to bulk RNA-seq to better understand the transcriptomic landscape of cholinergic macrophages under cold stress and their role in beige fat thermogenesis. Overall design: Comparison of ChAT-eGFP+ and ChAT-eGFP- macrophages FACS-isolated from IWAT of cold-exposed ChAT-eGFP reporter mice