Fly control using tactics that incorporate protein baits

The main aim of this project was to improve fruit fly control using tactics that incorporate protein baits. The spatial and temporal foraging patterns of <i>Bactrocera dorsalis</i>, <i>Ceratitis capitata</i> and <i>Ceratitis cosyra</i> for protein baits within a citrus tree with regard to fly age, sex and nutritional status were established in field cages. This was followed by the determination of protein and lipid body content of flies to assess their role played by nutrition on the likelihood of fly response to protein bait applied in the field. Upon completion of the two experiments of the first objective, a study that determined the response of the three pest species to protein baits applied at two different densities was carried out. Results showed that bait location in the canopy affects response by <i>B. dorsalis</i>, <i>C. capitata</i>, and <i>C. cosyra </i>with more flies foraging for protein in the middle to upper canopy heights of the tree compared to protein bait positioned on the lower canopy and directly on the ground. The first study also revealed that nutritional status, age and sex influenced protein bait response, with 1 day-old flies with prior access to protein showing a lower response to protein bait, while 10-day-old protein-deprived flies intensely foraged for protein regardless of sex or species. Females responded more to protein baits than males, and foraging for protein peaked in the warmest part of the day when temperatures were between 20-30℃ at a relative humidity ranging between 40-80%. Furthermore, the second study showed that species, diet history and bait density had a significant effect on the response to protein bait. <i>Ceratitis capitata </i>females were more attracted to the bait followed by <i>B. dorsalis, </i>and <i>C. cosyra</i> with the lowest response. Similar to results obtained in the first study, protein deprived females were most responsive to the bait regardless of species and bait density. Additionally, females were more attracted to 10 droplets of 200 µL protein bait applied in 10 traps than a single 2000 µL droplet applied in one trap regardless of diet and species. Based on the results, protein baits should be applied between the mid and upper canopy heights and should be evenly distributed on the tree canopy for increased effectiveness of control.The data is in folders that are partitioned into first and second objective, the first objective folder consists of both the data collected in from the field (field experiment) and nutrient assay data (nutrient assays). The second folder consists of data for the second experiment.