Single Cell RNA Sequencing of Young and Aged Murine Glioblastoma. Single Cell RNA Sequencing of Young and Aged Murine Glioblastoma

To understand the effects of aging on glioblastoma (GBM) immune microenvironment, differential gene expression of extra-tumral brain tissues between young and old mice were investigated by sc-RNASeq in this study. Overall design: C57BL/6 mice at the age of 8 weeks old and 93 weeks old were intracranially engrafted with either 1x10^3 CT2A-GFP-luc murine GBM cells or 1xPBS as sham control. Two weeks after tumor cell implantation, brain tissues from the non-tumor injection hemisphere (contralateral) were isolated and single cells were prepared using the Adult Brain Dissociation kit (Miltenyi Biotec) and subjected to sc-RNASeq.