16S rRNA amplicon sequencing reveals microbial community shifts during coastal wetland-to-farmland conversion in Qidong, Jiangsu Province

We sequenced the V4-1 region of 16S rRNA genes (primers 515F/806R) from 37 soil samples across 5 coastal wetlands and 4 farmlands in Qidong, Jiangsu (31.69-31.75N, 121.63-121.96E). This study aims to identify microbial responders to land-use change, linking diversity shifts to soil health in the Yangtze River Delta.