Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase. Genome-wide mapping of oxidative DNA damage via engineering of 8-oxoguanine DNA glycosylase

7,8-dihydro-8-oxoguanine (OG) is one of the major DNA oxidative damages, which has been concerned by researchers for its potential of mutagenesis and epigenetic regulation. Genome-wide mapping of oxidative DNA damage is of great value for understanding the biological impact of these modifications. Here we present a novel appoach to profile guanine oxidation in the mouse genome. Through structure-guided mutagenesis, we've successufully engineered 8-oxoguanine DNA glycosylase into a useful tool for OG detection. By enrichment of glycosylase-DNA complex, thousands of OG peaks were identified in the mouse genome. Overall design: Enrichment procedure was perform on mouse embryonic fibroblasts (MEFs) with two biological replicates. Genomic samples without any treatment were denoted as input; samples enriched by engineered glycosylase were denoted as enrich; samples pretreated with Fpg to remove oxidative damages before enrichment were denoted as control.