Additional file 1 of Evaluation of toxoplasmosis in pregnant women using dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein
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https://springernature.figshare.com/articles/dataset/Additional_file_1_of_Evaluation_of_toxoplasmosis_in_pregnant_women_using_dot-immunogold-silver_staining_with_recombinant_Toxoplasma_gondii_peroxiredoxin_protein/12991727/1
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Additional file 1: Fig. S1. SDS-PAGE analysis of pGEX-6P-1/TgPrx/BL21 expression products induced by different concentrations of IPTG. Fig. S2. SDS-PAGE analysis of pGEX-6P-1/TgPrx/BL21 expression products at different induction times. Fig. S3. SDS-PAGE analysis of optimized expression temperature of pGEX-6P-1/TgPrx/BL21. Fig. S4. The optimization of the type of blocking solution and the blocking time for Dot-IGSS assay. Fig. S5. The antibodies dilution for Dot-IGSS assay determined by checkerboard titration. Fig. S6. Detection of T. gondii infection in mice by rTgPrx-Dot-IGSS. Fig. S7. Detection of T. gondii infection in mice by Western blotting.
提供机构：
Zhuan-Zhuan Liu; Ren-Xian Tang; Kui-Yang Zheng
创建时间：
2020-09-23
