Additional file 1 of Evaluation of toxoplasmosis in pregnant women using dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein

Additional file 1: Fig. S1. SDS-PAGE analysis of pGEX-6P-1/TgPrx/BL21 expression products induced by different concentrations of IPTG. Fig. S2. SDS-PAGE analysis of pGEX-6P-1/TgPrx/BL21 expression products at different induction times. Fig. S3. SDS-PAGE analysis of optimized expression temperature of pGEX-6P-1/TgPrx/BL21. Fig. S4. The optimization of the type of blocking solution and the blocking time for Dot-IGSS assay. Fig. S5. The antibodies dilution for Dot-IGSS assay determined by checkerboard titration. Fig. S6. Detection of T. gondii infection in mice by rTgPrx-Dot-IGSS. Fig. S7. Detection of T. gondii infection in mice by Western blotting.