NOX3 complex:RAC1:GTP generates superoxide from oxygen

While NOX3:CYBA complex has constitutive NADPH oxidase activity, the presence of NCF1, NCF2 or NOXA1 and RAC1:GTP enhances the production of superoxide O2- by the NOX3:CYBA complex. When NCF1 is replaced with NOXO1, RAC1:GTP becomes dispensible for the full activation of the NOX3 complex (Ueno et al. 2005, Ueyama et al. 2006, Miyano and Sumimoto 2007, Kao et al. 2008)尽管NOX3:CYBA复合体具有构成性的NADPH氧化酶活性，但NCF1、NCF2或NOXA1以及RAC1:GTP的存在可增强NOX3:CYBA复合体产生超氧阴离子O2-的能力。当NCF1被NOXO1所替代时，RAC1:GTP对于NOX3复合体的完全激活变得非必需（Ueno等，2005年；Ueyama等，2006年；Miyano和Sumimoto，2007年；Kao等，2008年）