Quantification of aTS and iTS in parasites representing high and low TS activity producers.
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a) Parasites correspond to low (TcI) and high (TcII and TcVI) TS activity producer stocks as described by Risso et al[37].Parasite DNA was subjected to quantitative real time PCR and aTS/iTS presence was determined by using probes labeled with reporter dyes. Gene number per haploid genome was determined by Ct comparison with that obtained for the pvdh single copy gene. Ct: cycle of threshold; ND: not detectable.
创建时间:
2015-12-02



