RNA-seq of primary human macrophages uninfected or infected with Yersinia enterocolitica strains. RNA-seq of primary human macrophages uninfected or infected with Yersinia enterocolitica strains

Pathogenic bacteria Yersinia enterocolitica injects virulence plasmid-encoded effectors through the type three secretion system into macrophages to modulate gene expression. At this point there are no comprehensive gene expression analysis in primary human macrophages analyzing the effect of virulence plasmid-encoded factors on transcription. For this primary human macrophages were infected for 1.5 h and 6 h with mock, WAC (virulence plasmid-cured strain) or WA314 (wild type) and samples were subjected to RNA-seq. The effect of effector protein YopP on gene expression in macrophages was analyzed using a wild type strain lacking YopP.